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. 2011 Jan 13;6(1):e16180. doi: 10.1371/journal.pone.0016180

Figure 1. BRAFV600E suppresses p21 and p27 induction by RASSF1A in FRO cells.

Figure 1

(a) FRO Cells were transfected with HA-RASSF1A as indicated and incubated for 24 h. Total lysates were analyzed for p21 and p27 expression by Western blot analysis. (b) Lysates from FRO cells co-transfected with vectors for HA-RASSF1A and BRAF (wild type or the V600E mutant), as indicated, were subjected to Western blot analysis. (c) FRO cells transiently transfected with BRAFV600E and RASSF1A were incubated with MEK (U0126, 10 µM) or PI3K (wortmannin, 1 µM, Ly294002, 10 µM) inhibitors for 12 h as indicated. The lysates were subjected to western blot analysis with primary antibodies as indicated. (d) FRO cells co-transfected with RASSF1A (0.5 µg/well) and SiMST1 (20 µM/well Stealth™ RNA) were incubated for 24 hrs as indicated. Abbreviations: CTL; control.