Fig. 3. Analyses of p56^dok-2 expression and peritoneal cells in hr/hr mice. (A) Total RNA from bone marrow cells or spleen cells of 10- or 13-week-old hr/hr mice and heterozygous control (+/hr) mice was prepared, blotted to membrane (5 µg/lane for bone marrow cells and 2.5 µg/lane for spleen cells) and then hybridized with probe to p56^dok-2 or GAPDH. Exposure times: top panel, 3 days; bottom panel, 1 h. (B) FACS profiles of peritoneal cells from hr/hr and +/hr mice. Cells were stained with anti-Mac-1 antibody and analyzed by flow cytometry. (C) Wright–Giemsa-stained cytospin preparations of peritoneal cells collected from hr/hr and +/hr mice. Arrows indicate typical cells with macrophage morphology. (B and C) Data shown are representative of an analysis with 8 hr/hr mice and 8 +/hr mice 13 weeks old. The experiments were repeated using 10-week-old mice.