Fig. 4.
OII deactivation in C-1 channels is voltage-dependent. Holo OII states were formed (activation for 3 s at -140 mV or 2 s at -160 mV for C-1, and for 2 s at -160 mV for C-2), and deactivation time constants were measured at various holding voltages (subscript on τ symbol). Ratios of time constants from specified pairs of holding voltages provide voltage-dependence factors. Bars plot voltage-dependence factors (mean ± SD) and brackets with t test P values mark comparisons showing significant difference, showing attenuation of voltage dependence at positive voltages. See Fig. S2 for examples of holo C-1 current traces, and compiled data for apo C-1 and C-2.