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An altered form of Kdo2-lipid A in E. coli WBB06 expressing KdoO. Lipids were extracted (35) and separated by TLC, followed by charring with 10% sulfuric acid in ethanol. Cells harboring either pYpKdoO or pBaKdoO generated a slower migrating derivative of Kdo2-lipid A, consistent with Ko formation.
