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. 2010 Dec 27;108(2):786–791. doi: 10.1073/pnas.1011098108

Fig. 7.

Fig. 7.

TUC338 modulates cell growth in HCC cells. (A) HepG2 and Huh-7 cells were transfected with siRNAs against TUC338 or control siRNA for 48 h and then plated. After 24, 48, and 72 h, cells were counted by trypan blue staining. Mean values of three independent experiments with SEM are represented. *P < 0.05 compared with control. Cell viability after transfection with siRNA-1 was not significantly different from that with siRNA-2 (P > 0.05 at each time point in each of the cell lines). (B) HepG2 cells were transfected with siRNA-2 anti-TUC338 or control siRNA for 48 h, and analysis of cell-cycle distribution was performed by flow cytometry. Compared with controls, there was a reduction of 30% in S phase and of 12% in G2/M phase for cells transfected with siRNA against TUC338. Bars represent the mean and SEM of three experiments. *P < 0.05 compared with controls. (C) HepG2 cells were transfected with siRNA anti-TUC338 or control siRNA by nuclear transfection for 48 h and then plated in agar in 96-well plates. Anchorage-independent growth was assessed fluorometrically after 7 d. Bars represent the mean and SEM of two experiments performed in seven replicates. *P < 0.05.