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. 2010 Dec 27;108(2):786–791. doi: 10.1073/pnas.1011098108

Fig. 8.

Fig. 8.

TUC338 modulates cell growth in mouse hepatocytes. BNL-CL.2 are nonmalignant embryonic mouse hepatocytes. BNL-SVA.8 cells are derived from BNL-CL.2 after SV40 transformation, and they exhibit transformed cell growth. (A) TUC338 expression was assessed by real-time-PCR and normalized to that of RNU6. Bars represent the mean and SEM of three experiments. (B) BNL-CL.2 and BNL-SVA.8 were plated in soft agar, and colonies were counted after 4 wk. Representative pictures are shown along with the mean and SEM of three independent experiments. *P < 0.05. (C) BNL-CL.2 and BNL-SVA.8 cells were plated in 6-well plates, and cell number was counted by trypan blue staining at different time points. Mean and SEM derived from three independent experiments are shown. *P < 0.05 compared with BNL-CL.2. (D) BNL-SVA.8 cells were transfected with siRNA-1 anti-TUC338 or siRNA control for 48 h. Bars represent mean and SEM of four replicates. At the indicated times, cells were counted after trypan blue staining. Mean and SEM from three independent experiments are represented. *P < 0.05 compared with siRNA control.