Fig. 3.

Effect (mean ± SEM) of T cells and CD40L on SC osteoclastogenic activity and cytokine secretion. (A) Effect of ovx on the osteoclastogenic activity of SCs from mice treated with irrelevant isotype-matched Ab (Irr. Ig) or anti-CD4 and anti-CD8 Abs (CD4/8 Ig). All cultures were stimulated with PTH (1 nM), an agent that targets SCs. (B) Effect of ovx on the osteoclastogenic activity of SCs from WT and CD40L^−/− mice. All cultures were stimulated with PTH (1 nM). (C) Effect of T cells from sham-operated and ovx mice and of CD40L neutralization on the osteoclastogenic activity of SCs. All cultures were stimulated with PTH (1 nM). (D–F) Effect of ovx on the production of M-CSF, RANKL, and OPG by SCs from T cell-depleted mice, CD40L^−/− mice, and MR-1–treated mice. *P < 0.05 and **P < 0.01 compared with the corresponding controls. ^#P < 0.05 compared with the other groups.