Fig. 2.

Coimmunoprecipitation of DNA repair components with H3K36me2 after IR. (A) Immunoprecipitates (IP) of H3K36me2 were collected from HT1904 cells before (C) and 15 or 60 min after 10 Gy γ-radiation and analyzed by Western blot for NBS1, Ku70, DNA ligase IV; input, nonspecific IgG, and H3K36me2 controls are also shown. ChIP analysis of early DNA repair components at a single induced DSB. Increasing Metnase levels enhances the presence of Ku70 (B) and phospho-NBS1 (C) at the single DSB, while repressing Metnase reduced the presence of these proteins. Neither Ku70 nor phospho-NBS1 were detected at the target DSB site prior to DSB induction (B and C).