Fig. 3.

Dimethylation of H3K36 enhances DSB repair. (A) Repair of a single DSB measured by real-time quantitative PCR in cells over- or underexpressing Metnase; values are inverse percentages of input (uncut) DNA prior to DSB induction and normalized to GAPDH (input) controls. Statistics calculated at each time point for over- or underexpression vs. cognate empty vector controls. (B) As in A but with cells expressing wild-type or D248S (SET-defective) Metnase. (C and D) Overexpression of JHDM1a demethylase reduces H3K36me2 presence at an induced DSB (C) and reduces DSB repair (D).