Fig. 3.

AaMet and AaFISC are required for expression of JH target genes in the midgut of adult female mosquitoes. (A) Double-stranded RNA (dsRNA) induced gene knockdown. A 0.5-μg quantity of dsRNA for either AaMet or AaFISC was injected into newly emerged female mosquitoes within 30 min after eclosion. DsRNA for bacterial malE gene was used as control. Then, 4 d after injection, midguts were collected from the mosquitoes. Total RNA was extracted and subjected to quantitative RT-PCR analysis. Results are expressed as percentage of mRNA levels in the uninjected (UGAL) mosquitoes. (B) Schematic structure of the AaET gene. Three pairs of primers were designed to amplify the distal upstream region (ET2), the proximal promoter region (ETv), and the coding region of AaET (ETc6). Association of AaMet (C) and AaFISC (D) with the AaET promoter was measured by chromatin immunoprecipitation assays. Amount of immunoprecipitated DNA in each sample was represented as signal relative to the total amount of input chromatin. PBM, post blood meal; PE, posteclosion.