Table 1.
Bacterial strains and plasmids used
| Strain or plasmid | Genotype | Source/ref. |
|---|---|---|
| P. aeruginosa | ||
| PAO1 | Wild-type prototroph | 30 |
| PAOR3 | qscR mutant of PAO1, GmR | This study |
| E. coli | ||
| DH5α | F− φ80dlacZΔM15Δ(lacZYA-argF)U169endA1 recA1 hsdR17 deoR gyrA96 thi-1 relA1 supE44 | 31 |
| S17-1 | thi pro hsdR recA RP4-2 (Tet∷Mu) (Km∷Tn7) | 32 |
| CM830 | E. coli K-12 derivative | |
| Plasmids | ||
| pGmΩ1 | Broad-host-range vector containing the aacC1 gene, GmR | 33 |
| pCR2.1 | TA cloning vector, ApR | Invitrogen |
| pEX1.8 | Broad-host-range expression vector, ApR | 34 |
| pQF50 | Broad-host-range lacZ transcriptional fusion vector, ApR | 35 |
| pMP7 | E. coli cloning vector, (ColE1) ori, ApR | 36 |
| pKL13 | pMP7 containing 3.1 kb qscR fragment, ApR | This study |
| pKL14 | pKL13 with 1.6 kb aacC1 interrupting qscR ApRGmR | This study |
| pKV69 | Vector carrying mobRP4 CmRTcR | K. Visick, Loyola Univ., Chicago |
| pMW16 | pKL14 containing 1.6 kb mobRP4 from pKV69, ApRGmRCmR | This study |
| pKL8 | pCR2.1 containing 714 bp qscR fragment, ApR | This study |
| pKL9 | pEX1.8 containing ptac-qscR, ApR | This study |
| pMW303 | Broad-host-range phzABC-lacZ reporter, ApR | 15 |
| pMW301 | Broad-host-range hcnAB-lacZ reporter, ApR | 15 |
| pSC11 | Broad-host-range lasI-lacZ reporter, ApR | This study |
| pSC10 | Broad-host-range lasR-lacZ reporter, ApR | This study |
| pMW305 | Broad-host-range rhlI-lacZ reporter, GmR, SpR, SmR | 15 |
| pMW304 | Broad-host-range rhlR-lacZ reporter, GmR, SpR, SmR | 15 |
| pMW105B | Broad-host-range qsc105-lacZ reporter, ApR | This study |