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. Author manuscript; available in PMC: 2012 Jan 7.
Published in final edited form as: Cell Stem Cell. 2010 Dec 16;8(1):84–95. doi: 10.1016/j.stem.2010.11.026

Figure 3. EGFR signaling promotes ISC proliferation and midgut growth.

Figure 3

A. Ectopic ISC proliferation induced by Vn. Vn was induced in the midgut using the inducible VM-specific driver, 24Bts. B. ISC proliferation induced by activated EGFR signaling. Transgenes were induced in the midgut for 2 days using the esgtsF/O or MyoIAts system. Midguts were scored for PH3+ mitotic figures in both A and B. C-E. Adult midgut growth measured using the esgtsF/O system. Both sSpi (D) and λTOP (E) promoted significant new midgut cell formation. F-F”. RasV12 also promoted the formation of new mature midgut cells. Most of the newly formed large polyploid midgut cells (GFP+, arrows) were positive for mature EC marker, PDM-1.