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. Author manuscript; available in PMC: 2012 Jan 7.
Published in final edited form as: Cell Stem Cell. 2010 Dec 16;8(1):84–95. doi: 10.1016/j.stem.2010.11.026

Figure 6. Jak/Stat-induced ISC proliferation requires EGFR signaling.

Figure 6

A. ISC proliferation induced by EGFR and Jak/Stat signaling. With the exception of co-expressing sKrn and Upd in the ECs (MyoIAts>Upd + sKrn), all the other ectopic expression experiments were performed using the esgtsF/O driver. Midgut mitotic indices (PH3+) were quantified after activating the transgenes for 2 days. B-J. ISC clonal assay. GFP-marked ISC clones were induced using the MARCM system and analyzed 4 or 8 days later. The sizes of the ISC clones were indicated. Vn-induced ISC proliferation is dependent on Jak/Stat signaling (B-D). Activated Ras (RasV12)-induced ISC proliferation is independent of Jak/Stat signaling (F-G). Some EB clones overexpressing RasV12 underwent extra round of endoreplication (E). Upd-induced ISC proliferation is dependent on EGFR signaling (H-J). K. Quantification of ISC clone sizes. The sizes of ISC clones were measured 4 or 8 days after clone induction (ACI) using the MARCM system. L. RT-qPCR analysis of the induction of Jak/Stat and EGFR signalings by Pe infection in the absence of either pathway (esgtsF/O>Stat or Egfr RNAi).