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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Neurochem Int. 2010 Nov 27;58(2):176–179. doi: 10.1016/j.neuint.2010.11.012

Figure 1.

Figure 1

In cultures of a non-transfected C6 glioma cell line, application of sodium nitroprusside (SNP 100 μM) significantly increased cGMP formation. Basal levels of cGMP in C6 glioma cell line were stimulated almost 40-fold with 100 μM sodium nitroprusside (18 ± 0.4 to 704 ± 21.2 pg/ml, respectively). The group II mGluR agonist DCGIV (1, 10 100 μM) did not significantly change sodium nitroprusside-stimulated cGMP formation. Cells were grown on 96 wells, washed twice with Locke buffer, preincubated for 10 min at room temperature with the buffer containing phosphodiesterase inhibitor (300 mM IBMX) and incubated with agonists for 6 min. Results are expressed as means ± SEM (n = 6 cultures per treatment group). Statistical significance was determined by Student’s t-test in all figures. *P<0.05, **p<0.01