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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Neurochem Int. 2010 Nov 27;58(2):176–179. doi: 10.1016/j.neuint.2010.11.012

Figure 2.

Figure 2

A. rtPCR analysis of transcripts from non-transfected C6 cells (lanes 2 and 3) and two C6 cells stably transfected with mGluR2 (lanes 1 and 4). Major DNA bands in lanes 1 and 4 are in the size range predicted for mGluR2 rtPCR products obtained via the primers used in this study.

B. Western blot analysis of protein from non-transfected C6 cells (lanes 2 and 3), two C6 cell lines stably transfected with mGluR2 (lanes 1 and 4) and one C6 cell line stably transfected with mGluR3 (lane 5). Blots were reacted with mGluR2/3 antibody (Chemicon). Under these experimental conditions, mGluR2 receptors migrate more as dimers (~200 kD) than monomers (~100 kD) while mGluR3 tends to migrate primarily as the monomer (~100 kD).