Table 2.
Results from intravital fluochrome labeling
| OVX | OVX Vib | Sham | Sham Vib | OVX vs. sham | Vib vs. nonvib (both OVX and sham groups) | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mean | SD | Mean | SD | Mean | SD | Mean | SD | P | P | |
| Periosteal apposition | ||||||||||
| Absolute apposition bandwidth (mcm) | ||||||||||
| Calcein green (d0–d18) | 9.49 | 2.33 | 10.26 | 4.29 | 8.65 | 1.26 | 8.77 | 2.08 | >0.05 | >0.05 |
| Alizarin red (d18–d24) | 9.43 | 3.20 | 8.63 | 2.17 | 8.67 | 1.24 | 7.22 | 2.22 | >0.05 | >0.05 |
| Tetracycline (d24–d35) | 13.51 | 5.28 | 14.84 | 3.79 | 13.15 | 2.21 | 14.27 | 3.77 | >0.05 | >0.05 |
| Sum | 32.43 | 33.73 | 30.47 | 30.26 | ||||||
| Endosteal apposition | ||||||||||
| Absolute apposition bandwidth (mcm) | ||||||||||
| Calcein green (d0–d18) | No significant appositions | No significant appositions | 3.91 | 1.46 | 3.91 | 1.3 | <0.05 | >0.05 | ||
| Alizarin red (d18–d24) | 3.93 | 1.055 | 4.11 | 1.32 | <0.05 | >0.05 | ||||
| Tetracycline (d24–d35) | 3.97 | 1.47 | 3.54 | 1.48 | <0.05 | >0.05 | ||||
| Sum | 11.81 | 11.56 | ||||||||
The widths of apposition bands, calcein green, alizarin red, and tetracycline in cortical surface in subtrochanteric cross sections of rat femurs (15 mm distal from femoral head) were measured by fluorescence microscopy (400×). The P value of the difference between treated and untreated animals was calculated using a two-way ANOVA. P < 0.05 was considered significant. There are no interactions between OVX and vibration (Vib)