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. 2001 Feb 27;98(5):2758–2763. doi: 10.1073/pnas.051630298

Figure 1.

Figure 1

Differential expression genes identified by DD. (Left) RNAs prepared from noninfected (lane 1) and infected mouse brain at days 3–7 p.i. (lanes 2, 3, 4, 5, and 6, respectively) were analyzed as indicated in Materials and Methods: DD patterns of VL-30 (A), IRF7 (B), and RV N protein (C). Confirmation of up-regulation of VL-30 (D) and IRF7 (E) mRNAs by Northern blot. Total RNA prepared as in D and E was assayed with RV N-protein probe (F). The hybridization showed different patterns of expression for RV N protein (RVN) and RV genomic RNAs (GenRV). The blots D, E, and F were reprobed with G3PDH as a loading control. (G–J) PhosphorImage quantification of D, E, and F Northern blots. The quantifications were normalized according to G3PDH-hybridized band intensities. Vertical y axis on graphs shows the expression levels of mRNAs relative to noninfected samples at 3, 4, 5, 6, and 7 days p.i. (x axis): G, VL-30; H, IRF7; I, RV genomic; J, RV N protein.