TABLE 1.
Cra-binding sites on the E. coli genomea
| Target prediction | No. of binding sites |
|
|---|---|---|
| SELEX | Database | |
| SELEX | 144 | |
| SELEX + RegulonDB | 20 | 20 |
| RegulonDB | 3 | |
| Total | 164 | 23 |
a
A collection of DNA fragments isolated by genomic SELEX was subjected to SELEX chip analysis. A total of 194 peaks were identified in the SELEX chip pattern, 164 of which were located within spacer regions. Among 23 known Cra targets, 20 were included in this library, and thus, a total of 144 Cra-binding sites were newly identified. There were 167 predicted targets.