FIG. 1.
Amino acid sequence deduced from the A. pernix open reading frame (ORF) APE1583.1. Bold red letters identify the N and C termini of the OppAAp protein (pQ32 and T819). The N-terminal sequence, included in a green box, was obtained by LC-MS/MS analysis. The nicked peptide bond (E570-G571) is marked by a red asterisk. The N-terminal sequence of the 27-kDa fragment, obtained by Edman degradation analysis, is black boxed. The coverage sequence obtained by proteolysis is shown underneath the amino acid sequence as follows: blue lines indicate the peptides obtained by endoproteinase V8 hydrolysis, and red lines indicate peptides obtained by CNBr hydrolysis. Regions in gray and blue correspond to the 57-kDa and 27-kDa fragments, respectively.