FIG. 3.
SDS-PAGE analysis of extracellular and outer surface protein extracts from Aeropyrum pernix cells harvested at the middle exponential growth phase. Lane 1, molecular mass markers (broad range); lane 2, extracellular protein fraction; lane 3, membrane-surface protein fraction. OppAAp was identified from tryptic digests followed by LC-MS/MS analysis of several electrophoresis bands. A large amount of OppAAp was revealed in the 200-kDa band, indicated by bold arrows; the protein was also identified from different electrophoretic bands (dashed arrows).