. 2010 Nov 19;193(3):723–733. doi: 10.1128/JB.00708-10

TABLE 4.

Relative GTP levels before and after treatments and accumulation of (p)ppGpp in three genetic backgrounds^a

Treatment and strain	Treatment time (min)	GTP level (%)	Accumulation (nmol/A₆₉₀)
Treatment and strain	Treatment time (min)	GTP level (%)	ppGpp	pppGpp
SHX
relA⁺	0	100	0	0
relA⁺	15	20	0.58	0.75
relA	0	100	0	0
relA	15	170	0	0
relA(S)	0	100	0	0
relA(S)	15	83	0	0
αMG
relA⁺	0	100	0	0
relA⁺	15	22	0.42	0.43
relA	0	100	0	0
relA	15	86	0	0
relA(S)	0	100	0	0
relA(S)	15	25	0.11	0.16

The levels of GTP were calculated by densitometric scans of autoradiograms, The absolute levels of (p)ppGpp were determined by excision of the appropriate spots from TLC plates, followed by scintillation counting, performed as in a previous study (17).