TABLE 2.
Plasmids used in this study
| Designation | Description | Source or reference |
|---|---|---|
| pWB126 | difA(M49A) in pWB210; Kanr | This study |
| pWB200 | Mx8 phage ATT site; ccdB for lethal selection; Kanr | 47 |
| pWB210 | Full-length difA with promoter in pWB200; Kanr | 10 |
| pWB230 | pWB210 with 2 silent point mutations in difA (CGC to CGG at codon 34 to introduce an SmaI site and CTG to CTC at codon 151 to introduce an SacI site) | 10 |
| pHN001 | difA(E158D) in pWB230; Kanr | 10 |
| pHN002 | difA(Q394D) in pWB230; Kanr | 10 |
| pHN003 | difA(E400D) in pWB230; Kanr | 10 |
| pHN004 | difA(E407D) in pWB230; Kanr | 10 |
| pXQ707 | difA(E183D) in pWB230; Kanr | 10 |
| pXQ708 | difA(E428D) in pWB230; Kanr | 10 |
| pXQ709 | pWB210 with the −138GTC mutation; Kanr | This study |
| pXQ710 | pWB210 with the difA start codon GTG mutated to GTC; Kanr | This study |
| pXQ711 | pWB210 with the difA start codon GTG mutated to ATG; Kanr | This study |
| pXQ731 | difA(E183A) in pWB230; Kanr | This study |
| pXQ732 | difA(Q394A) in pWB230; Kanr | This study |
| pXQ733 | difA(E400A) in pWB230; Kanr | This study |
| pXQ734 | difA(E407A) in pWB230; Kanr | This study |
| pXQ735 | difA(E183Q) in pWB230; Kanr | This study |
| pXQ736 | difA(Q346E) in pWB230; Kanr | This study |
| pXQ737 | difA(E400Q) in pWB230; Kanr | This study |
| pXQ738 | difA(E407Q) in pWB230; Kanr | This study |
| pXQ739 | difA(Q346N) in pWB230; Kanr | This study |
| pXQ740 | difA(Q429D) in pWB230; Kanr | This study |
| pXQ742 | difA(E158A) in pWB230; Kanr | This study |
| pXQ743 | difA(E158Q) in pWB230; Kanr | This study |
| pXQ744 | difA(E428A) in pWB230; Kanr | This study |
| pXQ746 | difA(E428Q) in pWB230; Kanr | This study |
| pXQ747 | difA(Q429A) in pWB230; Kanr | This study |
| pXQ748 | difA(Q381E) in pWB230; Kanr | This study |
| pXQ749 | difA(Q381N) in pWB230; Kanr | This study |
| pXQ752 | difA (Δ2-40) in pWB230; Kanr | This study |
| pXQ754 | difA (ΔI) in pWB230; Kanr | This study |
| pXQ755 | difA (ΔIV) in pWB230; Kanr | This study |
| pXQ756 | difA [Δ(I+IV)] in pWB230; Kanr | This study |
| pXQ757 | difA (ΔII) in pWB230; Kanr | This study |
| pXQ758 | difA (ΔIII) in pWB230; Kanr | This study |
| pXQ759 | difA [Δ(II + III)] in pWB230; Kanr | This study |
| pXQ769 | difA (Δ10-35) in pWB230; Kanr | This study |
| pXQ770 | difA (Δ2-8) in pWB230; Kanr | This study |
| pXQ771 | difA (Δ9-18) in pWB230; Kanr | This study |
| pXQ772 | difA (Δ19-28) in pWB230; Kanr | This study |
| pXQ773 | difA (Δ29-38) in pWB230; Kanr | This study |