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EPS production of the mutants with single substitutions
| Residue | Result for substitutiona: |
||||
|---|---|---|---|---|---|
| D | Q | E | A | N | |
| E158 | + | + | NA | + | NA |
| E183 | +/− | + | NA | ++ | NA |
| Q394 | − | NA | − | + | − |
| E400 | +/− | +/− | NA | − | NA |
| E407 | + | ++ | NA | ++ | NA |
| E428 | + | + | NA | + | NA |
| Q429 | − | NA | ++ | ++ | + |
+, the strain produces a similar level of EPS to that of YZ619 (ΔdifA/difA+); −, no detectable EPS production; ++, EPS production is significantly increased over the wild type; +/−, EPS production is significantly reduced. NA, not applicable.
