Table 2.
QTLs identified for seed mass, germination and heterotrophic growth, (a) at sub-optimal temperatures in the LR5 population (Jemalong × F83005.5), (b) at 20°C, a supra-optimal temperature in the LR4 population (Jemalong × DZA315.16; two experiments SNES and LEG)
| Trait | QTLa | Chrb | QTL position (cM) | Support interval (cM) | Closest marker | Peak LOD score | R 2%c | Additive allele effecte |
|---|---|---|---|---|---|---|---|---|
| (a) | ||||||||
| SM (mg) | SM5-LR5 | 5 | 38 | 30–46 | MTE33 | 3.4 | 11.7 | −0.21 |
| SM8-LR5 | 8 | 80 | 78–82 | Mtic1194 | 3.1 | 10.9 | 0.26 | |
| R 2adjd = 21.2 | ||||||||
| T90 (h) | T90.8-LR5 | 8 | 8 | 4–10 | MTE118 | 3.0 | 15.0 | −24.20 |
| FPG (%) | FPG3-LR5 | 3 | 26 | 20–36 | MTE19 | 3.1 | 12.5 | −2.97 |
| HL (mm) | HL1-LR5 | 1 | 4 | 0–12 | MTE75 | 5.1 | 17.7 | −1.82 |
| HL2-LR5 | 2 | 6 | 2–12 | MTE10 | 6.7 | 22.0 | −2.10 | |
| HL8-LR5 | 8 | 66 | 62–70 | MTE136 | 8.0 | 25.5 | −2.00 | |
| R 2adjd = 59.4 | ||||||||
| HW (mm) | HW2-LR5 | 2 | 70 | 64–70 | MTE80 | 3.4 | 12.1 | 0.02 |
| Trait | QTLa | Chrb | QTL position (cM) | Support interval (cM) | Closest marker | Peak LOD score | R2%c | Additive allele effecte | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| (b) | |||||||||||||||
| Experiment | SNES | LEG | SNES | LEG | SNES | LEG | SNES | LEG | SNES | LEG | SNES | LEG | SNES | LEG | |
| SM (mg) | SM1-LR4 | 1 | 1 | 52 | 56 | 50–60 | 50–60 | MTE62 | MTE62 | 3.4 | 2.8 | 8.5 | 11.4 | 0.11 | 0.15 |
| SM5-LR4 | 5 | 2 | 0–8 | MTE30 | 3.0 | 8.0 | 0.11 | ||||||||
| SM7-LR4 | 7 | 7 | 52 | 48 | 48–58 | 42–52 | Mtic432 | Mtic432 | 7.6 | 2.7 | 18.5 | 10.8 | −0.18 | −0.15 | |
| SM8-LR4 | 8 | 70 | 66–76 | MTE56 | 3.4 | 8.6 | 0.11 | ||||||||
| R 2adjd = 35.4 | R 2adjd = 20.2 | ||||||||||||||
| IR (mm2/h) | IR4-LR4 | 4 | 44 | 34–48 | MTE29 | 3.1 | 7.9 | −6.31 | |||||||
| T20 (h) | T20.5-LR4 | 5 | 8 | 0–12 | MTE31 | 4.9 | 12.3 | 0.44 | |||||||
| T50 (h) | T50.5-LR4 | 5 | 8 | 0–14 | MTE31 | 4.6 | 11.6 | 0.59 | |||||||
| T70 (h) | T70.5-LR4 | 5 | 48 | 36–52 | MTE35 | 2.7 | 6.9 | 0.70 | |||||||
| T90 (h) | T90.5-LR4 | 5 | 82 | 74–86 | MTE37 | 2.9 | 19.9 | 2.81 | |||||||
| T90.7-LR4 | 7 | 62 | 58–66 | MTE125 | 2.9 | 20.6 | −3.04 | ||||||||
| R 2adjd = 38.7 | |||||||||||||||
| ELR (mm/h) | ELR3-LR4 | 3 | 54 | 48–58 | MTE20 | 3.3 | 8.5 | 0.03 | |||||||
SM seed mass, T20–T90 times to reach 20–90% of germination, FPG final percentage of germination, IR imbibition rate, HL final hypocotyl length, HW final hypocotyl width, ELR early embryonic axis elongation rate
aQTL designation
bChromosome
cProportion of the phenotypic variation accounted for each QTL
dProportion of the phenotypic variation explained by all QTLs
eAdditive allele effect is positive when the F83005.5 alleles or the DZA315.16 alleles increase the trait value in the LR5 or LR4 genetic backgrounds, respectively