Figure 1.

Expression of bimodal imaging transgenes using lentiviral vectors. A, A self-inactivating lentiviral system based on HIV-1 (CS-CGW) was used to construct the following vectors: fusion between GFP and Fluc, GFP and Rluc, Fluc and DsRed2, and Rluc and DsRed2 under the CMV promoter. B, hNSCs and glioma cells were transduced in culture with these lentiviral vectors at MOI = 1 and visualized for GFP or DsRed2 fluorescence. Magnification, 20×.