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. 2001 Feb 20;98(5):2770–2775. doi: 10.1073/pnas.051623398

Figure 1.

Figure 1

Analysis of monocistronic mRNAs containing the transcribed leader sequences of dendritically localized mRNAs. (A) Schematic illustration of monocistronic reporter mRNAs containing the transcribed leader sequences (TLS) 5′ of the Photinus luciferase gene. (B) Northern blot analysis of monocistronic mRNAs using equivalent amounts of total RNA from B104 cells transfected with the monocistronic constructs indicated and probed with a radiolabeled cRNA to the Photinus luciferase gene. (C) Photinus luciferase activity generated by monocistronic reporter mRNAs. Luciferase activity was normalized to that generated by the parent vector PM. (D) Monocistronic constructs were cotransfected with either a plasmid expressing hypophosphorylated 4E-BP1 or control plasmid and assayed for luciferase activity. The activities obtained in cells cotransfected with the mutated 4E-BP1 is represented as a percentage of the activity obtained in cells cotransfected with the control plasmid.