Table 1.

Kinetic paramerters for NPG binding to LacY measured by stopped-flow at different pH using direct sugar binding method based on Trp→NPG FRET (31)

pH	koff, s−1	kon, M−1 s−1	Kdapp, μM
5.5	35	0.50 × 106	70
7.5	18	0.43 × 106	42
9.5	114	0.50 × 106	228