Table 1.
Bovine milk casein phosphopeptides identified by hierarchical data analysis.
| Casein Isoform |
Peptide Sequence | # of Phos |
Expt. Mass | Calc. Mass | Method | Ref. | |
|---|---|---|---|---|---|---|---|
| α-S1 |
58DIGpSESTEDQAMEDIK73 or 58DIGSEpSTEDQAMEDIK73 |
1 | 1847.7281 | (+2) | 1847.7223 | Zr, Ti | [26, 27, 40, 60] |
| 58DIGpSEpSTEDQAMEDIK73 | 2 | 1927.6937 | (+2) | 1927.6916 | Zr, Ti | [26, 60, 71, 72] | |
| 1927.6919 | (+3) | 1927.6916 | Zr, Ti | ||||
| 58DIGpSEpSpTEDQAMEDIK73 | 3 | 2008.6677 | (+2) | 2007.6580 | Zr | [26] | |
| 2029.6459 | (+2) | 2029.6399 | Zr, Ti | ||||
| 52VNELpSK57 | 1 | 769.34972 | (+2) | 769.34915 | Zr | [27], [40, 60] | |
| 50EKVNELpSK57 | 1 | 1026.4879 | (+2) | 1026.4868 | Zr | [27], [40, 60] | |
| 1048.4691 | (+2) | 1048.4687b | Zr | [27, 40, 60] | |||
| 50VNELSKDIGpSEpSTEDQAMEDIK57 | 2 | 2599.0676 | (+3) | 2598.0566a | Zr, Ti | [27, 40, 60] | |
| 50VNELpSKDIGpSEpSTEDQAMEDIK57 | 3 | 2679.0326 | (+3) | 2678.0229a | Zr, Ti | [27, 60] | |
| 2679.0315 | (+2) | 2678.0229a | Zr, Ti | ||||
| 121VPQLEIVPNpSAEER134 | 1 | 1682.7761 | (+3) | 1682.7775b | Zr, Ti | [26, 27, 40, 58, 60, 71, 72] | |
| 1660.7955 | (+2) | 1660.7942 | Zr, Ti | ||||
| 119YKVPQLEIVPNpSAEER134 | 1 | 1951.9575 | (+2) | 1951.9525 | Zr, Ti | [26, 27, 40, 60, 71, 72] | |
| 1951.9544 | (+3) | 1951.9525 | Zr, Ti | ||||
| 118KYKVPQLEIVPNpSAEER134 | 1 | 2102.0270 | (+3) | 2102.0295b | Ti | [27], [26, 60] | |
| 74QMEAEpSISpSpSEEIVPNpSVEQK94 | 4 | 2662.9280 | (+3) | 2662.9286b | Zr, Ti | [27], [26, 60] | |
| 74QMEAEpSIpSpSpSEEIVPNpSVEQK94 | 5 | 2743.9004 | (+3) | 2742.8950b | Zr | [27], [26, 60, 71, 72] | |
| α-S2 | 17NTMEHVpSpSpSEESIIpSQETYKQEK39 | 4 | 3005.1161 | (+3) | 3004.1010a | Zr, Ti | [27], [26, 60, 71, 72] |
| 39NMAINPpSKENLCSTFCK55 | 1 | 1979.8247 | (+3) | 1979.8425 | Zr | - | |
| 61NANEEEYSIGpSpSpSEEpSAEVATEEVK85 | 4 | 3008.59 | (+2) | 3008.0295c | Zr | [26, 60, 72] | |
| 61NANEEEYSIGpSpSpSEEpSAEVATEEVK85 | 4 | 3031.0155 | (+2) | 3030.0115a,b | Ti | [26, 60, 71, 72] | |
| 3030.0242 | (+3) | 3030.0116b | Zr, Ti | ||||
| 152KTVDMEpSTEVFTK164 | 1 | 1722.8033 | (+3) | 1722.8020 | Zr, Ti | [26, 60, 71] | |
| 153TVDMEpSTEVFTKK165 | 1 | 1594.7097 | (+2) | 1594.7071 | Zr, Ti | [27, 60, 71] | |
| 153TVDMEpSTEVFTK164 | 1 | 1466.6127 | (+2) | 1466.6121 | Zr, Ti | [27, 60, 71] | |
| 141EQLpSTpSEENSK151 | 2 | 1539.6015 | (+2) | 1539.5976 | Zr | [27], [26, 60, 72] | |
| β | 48FQpSEEQQQTEDELQDK 63 | 1 | 2061.8303 | (+2) | 2061.8285 | Zr, Ti | [26, 27, 40, 60, 72] |
| 2061.8300 | (+3) | 2061.8285 | Zr, Ti | ||||
| 48FQpSEEQQQpTEDELQDK 63 | 2 | 2142.7993 | (+2) | 2141.7940a | Zr | - | |
| 45IEKFQpSEEQQQTEDELQDK63 | 1 | 2433.0548 | (+3) | 2432.0505a | Zr, Ti | [27], [26, 60] | |
| 44KIEKFQpSEEQQQTEDELQDK63 | 1 | 2561.1506 | (+3) | 2560.1445a | Ti | [27], [26, 60, 72] | |
a
Isotopic Precursor
b
[M+Na]+