Fig. 4.

Time course of PKC levels in the cortex and thalamus of P7 rats after 2-, 4- and 6-hour exposures to propofol. Whole-cell extracts were used to detect the presence of the 82-kDa intact PKC protein and the calpain-produced 36-kDa PKC fragment in the cortex (a) and the thalamus (b). Bars represent quantitative densitometric evaluation of the intact 36-kDa protein in the cortex (c) and the thalamus (d). Results are presented for animals at different recovery time points (0, 4, 16 and 24 h) after exposure to propofol for 2, 4 and 6 h. Representative immunoblots of the 6-hour treatment are shown. β-Actin was run as an internal standard for equal loading. The results are the means ± SEM. ^a p < 0.05 vs. control value presented as a black line, ^b p < 0.05 between treatments.