Figure 4. CIB1 augments PMA-induced ploidy in Dami cells.

A) Unstimulated CIB1oe and mock cells stained with Wright-Giemsa (arrows indicate multilobated nuclei). B) Representative flow cytometric histograms of DNA content of PMA and vehicle treated mock and CIB1oe cells. C) Western blot of lysates from Dami cells transfected with either CIB1 shRNA (CIB1^sh) or empty vector (Mock^sh) and probed with CIB1 and HSC-70. D) Representative flow cytometic histograms of DNA content of 3 day PMA treated CIB1^sh and Mock^sh cells.