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. 2011 Jan 14;6(1):e14527. doi: 10.1371/journal.pone.0014527

Figure 1. Selective inhibition of FasL and TRAIL or TRAIL-induced signaling and apoptosis.

Figure 1

In panel A, isogenic BJAB cells expressing GFP or GFP-FADD-DD and FADD-DDV108E were treated with increasing doses of Fas ligand, TRAIL, or agonistic anti-DR4 and anti-DR5 antibodies. The differences in responses indicate that both FADD-DD expressing cell lines were resistant to all TRAIL R targeted drugs, but that only FADD-DD expressing cells are resistant to FasL. In panel B, DISC IP experiments precipitating Fas or DR5 followed by western blotting for casp-8 or FADD demonstrate an increased recruitment of FADD-DD in place of endogenous FADD to the receptors. Panel C, illustrates that on activation of other signaling pathways leading to IkB degradation, and JNK phosphorylation, FADD-DD blocks signaling. This block leads to these events for both FasL and TRAIL, and that FADD-DD V108E blocks signaling only for TRAIL.