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. 2011 Jan 14;6(1):e15942. doi: 10.1371/journal.pone.0015942

Figure 3. Dosage effects of proteasomal inhibitors on p66Shc protein levels.

Figure 3

LNCaP C-33 cells were seeded in regular culture medium for 72 h, steroid starved for 48 h and then cells were treated with or without 10 nM DHT or different concentrations of (A) MG132 (0.01 to 10 µM), (B) lactacystin (0.1 to 10 µM) and (C) leupeptin (1 to 50 µM) for 24 h. Cells were harvested and lysates were analyzed by western blotting with Abs against total Shc protein and β-actin protein, respectively. The level of β-actin protein was detected as a loading control. The figure is a representative of at least five sets of independent experiments.