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. 2011 Jan 14;6(1):e15942. doi: 10.1371/journal.pone.0015942

Figure 5. Effects of E2 and proteasomal inhibitors on p66Shc protein levels in ovarian carcinoma cells.

Figure 5

CaOV-3 and OVCAR-3 cells were plated in regular medium for 72 h and steroid starved for 48 h. (A) CaOV-3 cells were treated with or without E2 (10 nM), MG 132 (0.01, 0.1 µM) or Lactacystin (0.1, 0.5 µM) for 48 h and (B) OVCAR-3 cells were treated with or without E2 (10 nM), MG 132 (0.01, 0.1 µM) or Lactacystin (0.05, 0.1 µM) for 48 h. Cells were harvested and lysates were analyzed by western blotting with Abs against total Shc protein and β-actin protein, respectively. The level of β-actin protein was detected as a loading control. The figure is a representative of three sets of independent experiments.