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. 2011 Jan 14;6(1):e16121. doi: 10.1371/journal.pone.0016121

Figure 2. TRIM5α orthologue expression in 293T cells and antiviral spectrum of TRIM5α orthologues against lentiviral production.

Figure 2

(A) Verification of the proper expression of TRIM5α orthologues. Control plasmid (−), TRIM5αhu (Hu), TRIM5αrh (Rh), TRIM5αag (Ag) and TRIM5αcy (Cy) expression in transfected 293T cells were verified via immunoblot analysis. Arrow depicts approximate TRIM5α band size. (B) 293T cells were co-transfected with a primate lentivirus proviral plasmid and increasing amounts of human (Hu), rhesus monkey (Rh), African green monkey (Ag) or cynomolgus monkey (Cy) TRIM5α-expressing plasmids. As infectious proviral plasmids, HIV-1 Group M (pNL4-3 and p89.6), HIV-1 Group O (pCMO2.41, pCMO2.5), HIV-2 (pROD10), and SIV (pSIVMAC1A11, pSIVAGMTan-1 and pSIVAGMSAB-1) were used. Viral titers were determined in GHOST(3)R3X4R5 indicator cells and described as infectious units per ml (IU/ml). Error bars indicate one standard deviation.