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. 2011 Jan 14;6(1):e16121. doi: 10.1371/journal.pone.0016121

Figure 4. Antiviral activities of chimeric TRIM5α proteins against HIV-1 production.

Figure 4

(A) Schematic representation of the chimeric TRIM5α constructs between human (Hu), rhesus monkey (Rh), African green monkey (Ag) and cynomolgus monkey (Cy) TRIM5α proteins. (B) Upper panel: late-restriction activities of chimeric TRIM5α proteins upon co-transfection with pNL4-3 into 293T cells. Viral titers were determined in GHOST(3)R3X4R5 indicator cells and reported as infectious units per ml (IU/ml). Error bars indicate one standard deviation. Lower panel: western blot analysis of chimeric TRIM5α proteins following transfection into 293T cells. Arrow indicates approximate full-length TRIM5α size.