Figure 4.

Dexras1 colocalization with markers and terminal deoxynucleotidyl transferase deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) labeling after light exposure. Immunofluorescence was performed to observe Dexras1 colocalization in the retina at 1 day after light exposure. The neuronal nuclei (NeuN) was used here as the marker of ganglion cells. A-C: Dexras1 immunoreactivity overlapped with NeuN-positive neurons in the retina. D-F: Cleaved caspase-3 as an apoptotic marker was colocalized in Dexras1-positive cells in the retina. C, F: Merged images. Magnification, 400×. TUNEL results were observed in sections of G: control retina, H: the normal retina, and I: the injured retina at 1 day after light exposure. In the control and normal retina, there were no TUNEL-positive cells. In the injured retina at 1 day after light exposure, TUNEL-positive labeling was apparent in the ONL, INL, and GCL. The scale bar is 50 µm in G-I. Abbreviations: GCL represents ganglion cell layer; INL represents inner nuclear layer; ONL represents outer nuclear layer.