Table 1. RT-PCR primer pairs.
| Name | Primer sequence | Melting temp (°C) | Product size (bp) |
|---|---|---|---|
|
HPRT1 for |
GACCAGTCAACAGGGGACAT |
61.8 |
194 |
|
HPRT1 rev |
ACACTTCGTGGGGTCCTTTT |
61.0 |
|
|
TNFα for |
CCCGAGTGACAAGCCTGTAG |
62.0 |
151 |
|
TNFα rev |
GAGGTACAGGCCCTCTGATG |
62.0 |
|
|
IL1β for |
GGGCCTCAAGGAAAAGAATC |
58.3 |
205 |
|
IL1β rev |
TTCTGCTTGAGAGGTGCTGA |
59.5 |
|
|
IL6 for |
AGGAGACTTGCCTGGTGAAA |
60.3 |
180 |
|
IL6 rev |
CAGGGGTGGTTATTGCATCT |
58.6 |
|
|
IL8 for |
AGGGTTGCCAGATGCAATAC |
58.3 |
213 |
|
IL8 rev |
CCTTGGCCTCAATTTTGCTA |
56.4 |
|
|
CXCL1 for |
GAAAGCTTGCCTCAATCCTG |
57.5 |
214 |
|
CXCL1 rev |
TCCTAAGCGATGCTCAAACA |
56.7 |
|
|
CXCL6 for |
GGGAAGCAAGTTTGTCTGGA |
59.4 |
237 |
| CXCL6 rev | CTTTCCCCCACACTCTTCAA | 59.8 |