Table 1. Polymerase chain reaction primers and product sizes of human CRYBB2 genes.
| Name | Primer sequence (5′-3′) | Tm (°C) | Product size (bp) |
|---|---|---|---|
| CRYBB22F |
5′-CCAGGTCCTCACTGCTGCTTC-3′ |
60.34 |
205 |
| CRYBB22R |
5′-CCCATTTTACAGAAGGGCAAC-3′ |
58.01 |
|
| CRYBB23F |
5′-ACCCTTCAGCATCCTTTGG-3′ |
57.56 |
314 |
| CRYBB23R |
5′-GCAGACAGGAGCAAGGGTAG-3′ |
61.9 |
|
| CRYBB24F |
5′-GCTTGGAGTGGAACTGACCTG-3′ |
61.92 |
244 |
| CRYBB24R |
5′-GGCAGAGAGAAAGTAGGATGATG-3′ |
61.98 |
|
| CRYBB25F |
5′-GCCCCCTCACCCATACTC-3′ |
61.86 |
242 |
| CRYBB25R |
5′-CCCCAGAGTCTCAGTTTCCTG-3′ |
61.92 |
|
| CRYBB26F |
5′CCTAGTGGCTTATGGATGCTC-3′ |
59.97 |
347 |
| CRYBB26R | 5′-TCTTCACTTGGAGGTCTGGAG-3′ | 59.97 |