MicroCT images of the distal femur in euploid and trisomic mice at the ages of 6 and 16 weeks (A). Analysis revealed a significant reduction in percent bone volume (B) and number of trabeculae (C), as well as a significant increase in trabecular separation (D) at both time points in trisomic mice. Although smaller, no significant difference was found in trabecular thickness (E) between the two groups. (Eu 6 wks, n= 12; Ts 6 wks, n= 10; Eu 16 wks, n= 10; Ts 16 wks, n= 16) (*p ≤ 0.05, **p ≤ 0.01, *** p ≤ 0.001)