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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: Anal Chem. 2010 Nov 29;83(2):463–466. doi: 10.1021/ac1025896

Scheme 1.

Scheme 1

Principle of 3′ → 5′ exonuclease activity assay. i) ExoIII digests DNA from the 3′-terminus, but is halted at the loop region; ii) The released guanine-rich sequence folds into a G-quadruplex; iii) G-quadruplex-selective probe crystal violet (CV) exhibits a “turn-on” emission response.