Figure 1.

(A) Schematic of the NSOM-FCS experiment. (B) Image of single molecules embedded in a polymer film obtained by a 120-nm aperture probe. (Red and green) Orthogonal polarization detection. Gaussian fitting of the line profile provides full width at half-maximum of 125 ± 9 nm. (C) Fluorescence intensity of a 100-nm bead in air as a function of the sample-probe distance. Aperture probe diameter was 120 nm.