Table 1.
Description | Source | |
---|---|---|
Strains | ||
B. bacteriovorus HD100-S1 | Streptomycin resistant wild-type of HD100 (DSM50701) prey-dependent | BfR strain collectiona |
B. bacteriovorus M1 | Saprophytic HD100 mutant strain | This study |
B. bacteriovorus M1.1 | Axenic HD100 mutant strain | This study |
E. coli K-12 | DSM423 | |
E. coli JC3272 | K-12, streptomycin resistant | [7] |
E. coli K-12 S17.1λpir | RecA thio, hsdR − M, Integrated plasmid RP4-2-Kn::Tn7/TC::M | DSM9526 |
E. coli K-12 Gene Hogs® | F-mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG fhuA::IS2 | Invitrogen, Darmstadt, Germany |
Plasmids | ||
pSUP202 | pMB1 replicon, ApR, TcR, CMR, mob | [15] |
pSUP404.2 | p15A replicon, RSF1010 replicon, KnR, CmR, mob | [9] |
pGFPuv | pMB1 replicon, ApR, gfpuv | Clontech (Mountain View, USA) |
pBR329 | pMB1 replicon; ApR, TcR, CmR | [3] |
pIV2 | p29807 replicon, KnR | [17] |
pNR24 | Insertion of Plac and gfp uv into pSUP404.2 via the restriction sites NheI and HindIII | This study |
aSpontaneous mutant isolated by Dominik Schwudke (pers. communication)