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. 2010 Sep 8;62(2):589–596. doi: 10.1007/s00284-010-9748-5

Table 3.

Selected singular restriction sites in the plasmid pSUP404.2 useful for cloning

Restriction enzyme Restriction site Gene/target
AatII 7824 Plasmid backbone
AsiSI 10053 KnR
BanII 9708 KnR
BmtI 9292 Plasmid backbone
Bsu36I 7400 Plasmid backbone
ClaI 9744 KnR
EcoRI 6745 CmR
HincII 254 Plasmid backbone
HindIII 10171 KnR
MluI 7744 Plasmid backbone
NaeI 399 Plasmid backbone
NheI 9288 Plasmid backbone
NruI 9710 KnR
PvuI 10053 KnR
SmaI 9927 KnR
XhoI 9651 KnR
XmaI 9925 KnR