Table 5.
Time | Probea | ∅ Ct-valueb | DNA molecules | Mean of DNA molecules | Ratio chromosome:plasmid |
---|---|---|---|---|---|
1 h | Chlor-I | 18.81 | 2.90 × 106 | 2.96 × 106 | |
Chlor-II | 19.83 | 3.05 × 106 | 1:6.96 | ||
Hit-I | 18.36 | 4.15 × 105 | 4.25 × 105 | ||
Hit-II | 19.19 | 4.34 × 105 | |||
2 h | Chlor-I | 18.74 | 2.89 × 106 | 3.01 × 106 | |
Chlor-II | 20.3 | 3.13 × 106 | 1:6.95 | ||
Hit-I | 18.65 | 4.21 × 105 | 4.33 × 105 | ||
Hit-II | 19.71 | 4.45 × 105 | |||
3 h | Chlor-I | 19.30 | 2.93 × 106 | 3.06 × 106 | |
Chlor-II | 20.63 | 3.18 × 106 | 1:7.08 | ||
Hit-I | 18.54 | 4.19 × 105 | 4.32 × 105 | ||
Hit-II | 19.67 | 4.45 × 105 | |||
4 h | Chlor-I | 18.65 | 2.87 × 106 | 3.01 × 106 | |
Chlor-II | 20.39 | 3.14 × 106 | 1:7 | ||
Hit-I | 18.16 | 4.10 × 105 | 4.30 × 105 | ||
Hit-II | 19.88 | 4.49 × 105 | |||
5 h | Chlor-I | 17.25 | 2.66 × 106 | 2.76 × 106 | |
Chlor-II | 18.48 | 2.85 × 106 | 1:6.78 | ||
Hit-I | 17.46 | 3.95 × 105 | 4.07 × 105 | ||
Hit-II | 18.53 | 4.19 × 105 | |||
21.5 h | Chlor-I | 13.56 | 2.09 × 106 | 2.08 × 106 | |
Hit-I | 13.68 | 2.07 × 106 | 1:6.75 | ||
Chlor-II | 13.43 | 3.09 × 105 | 3.08 × 105 | ||
Hit-II | 13.55 | 3.06 × 105 | |||
∅ | Mean value | 1:6.9 |
aTwo independent sets of experiments were performed, indicated by Roman numerals I and II
bEach Ct value was determined in quadruplicate