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. 2011 Feb;43(2):346–350. doi: 10.1016/j.soilbio.2010.10.022

Table 1.

General plant primers targeting plastid rbcL and trnL DNA, as well as specific primers for wheat (Triticum aestivum, TA) and maize (Zea mays, ZM) developed for this study. Columns show the primer name (forward followed by reverse primer), plastid DNA region, primer sequence and expected product size.

Primer name Plastid DNA region Sequence (5′–3′) Size (bp)
rbcL19a rbcL gene AGATTCCGCAGCCACTGCAGCCCCTGCTTC 153
rbcLZ1a rbcL gene ATGTCACCACAAACAGAGACTAAAGCAAGT
trnL-cb trnL exon CGAAATCGGTAGACGCTACG 647
trnL-db trnL exon GGGGATAGAGGGACTTGAAC
trnL-gb trnL (UAA) intron GGGCAATCCTGAGCCAA 91 (wheat)
trnL-hb trnL (UAA) intron CCATTGAGTCTCTGCACCTATC 89 (maize)
TA-s506 trnL CGGGAGGTGGGGGAGAT 228
TA-a508 trnL TTTCCCTCTTCTTTGAGACMAG
ZM-s510 trnL ATTTGATCATTATATACATTTTTGAGAT 94
ZM-a514 trnL TATGATTCTATCTTCTCCTTAGTGAAC