Table 1.
General plant primers targeting plastid rbcL and trnL DNA, as well as specific primers for wheat (Triticum aestivum, TA) and maize (Zea mays, ZM) developed for this study. Columns show the primer name (forward followed by reverse primer), plastid DNA region, primer sequence and expected product size.
| Primer name | Plastid DNA region | Sequence (5′–3′) | Size (bp) |
|---|---|---|---|
| rbcL19a | rbcL gene | AGATTCCGCAGCCACTGCAGCCCCTGCTTC | 153 |
| rbcLZ1a | rbcL gene | ATGTCACCACAAACAGAGACTAAAGCAAGT | |
| trnL-cb | trnL exon | CGAAATCGGTAGACGCTACG | 647 |
| trnL-db | trnL exon | GGGGATAGAGGGACTTGAAC | |
| trnL-gb | trnL (UAA) intron | GGGCAATCCTGAGCCAA | 91 (wheat) |
| trnL-hb | trnL (UAA) intron | CCATTGAGTCTCTGCACCTATC | 89 (maize) |
| TA-s506 | trnL | CGGGAGGTGGGGGAGAT | 228 |
| TA-a508 | trnL | TTTCCCTCTTCTTTGAGACMAG | |
| ZM-s510 | trnL | ATTTGATCATTATATACATTTTTGAGAT | 94 |
| ZM-a514 | trnL | TATGATTCTATCTTCTCCTTAGTGAAC |
From Poinar et al. (1998).
From Taberlet et al. (2007).