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. 2010 Dec 31;42(4):225–234. doi: 10.4143/crt.2010.42.4.225

Fig. 1.

Fig. 1

AMD3100 and T140 inhibit the chemotaxis of myeloma cells induced by stromal cell-derived factor-1 (SDF-1) and induce the internalization of surface CXCR4 in myeloma cells. (A) The myeloma cell lines RPMI8226 and U266 and CD138+ primary bone marrow myeloma cells were loaded into the upper chamber of a 24-well Transwell plate and were allowed to migrate into the lower chamber containing 100 ng/mL SDF-1 for 4 hours. AMD3100 and T140 were added at 10-5 M and 10-6 M in the upper chamber, respectively. The data are the mean±SD of the migration index from three independent experiments. (B) U266 cells were incubated with or without 10-5 M AMD3100 and 10-6 M T140, respectively, for 3 hours and then subjected to flow cytometry. To detect cytoplasmic CXCR4, the cells were permeabilized with saponin-based reagents before labeling. *p<0.05 compared to the controls (migration toward SDF-1).