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Transfection efficiency of PPA/DNA nanoparticles (N/P = 10) with or without chloroquine (CQ). Trancfection was conducted in HEK293 cells at a density of 140 K/well. After 4 h of transfection, medium containing 50 μm CQ was replenished and cells were incubated for 2 days before luciferase assay.
