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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Cancer Res. 2010 Sep 21;70(21):8792–8801. doi: 10.1158/0008-5472.CAN-08-4481

Figure 2.

Figure 2

Myeloid expansion in mice expressing PML-RARα and Dnmt3a1. Graphs represent fold differences in percentage of cells in mice analyzed compared with their experimental control (either WT or Dnmt3a1) from five independent experiments. Based on the expression of specific cell surface markers, cells of the bone marrow and spleen were classified into the following populations: (a) hematopoietic stem and progenitors (KLS+; c-kit+/Lin−/Sca1+); (b) HSCs (c-kit+/Lin−/Sca1+/Flk2−/CD34−); (c) MPPs (c-kit+/Lin−/Sca1+/Flk2+/CD34+); (d) myeloid progenitors (MP; c-kit+/Lin−/Sca1−); (e) granulocytes (Gr1+/Mac1+); (f) myeloid precursors (Mac1+/c-kit+); (g) B cells (B220+/CD19+); and (h) T cells (CD4+ or CD8+). MP cells were further subdivided into CMPs (CD34+/FcγR−), granulocyte/macrophage progenitors (GMP; CD34+/FcγR+), and megakaryocyte/erythrocyte progenitors (MEP; CD34−/FcγR−). Fold difference in the percentage of cells in each compartment is calculated based on the following formula: fold difference = (Exp %)/(Control %). Statistical analysis was performed using Kruskal-Wallis test. *, P < 0.05.