Figure 2. IL-6 colocalizes with macrophages from uninfected but not Helicobacter-infected mice.
Representative whole mounts of intestinal muscle isolated from uninfected or infected mice were stained with F4/80 (green), IL-6 (red), both F4/80 and IL-6, or appropriate isotype control. Colocalization of F4/80 and IL-6 expression is illustrated in orange. Photomicrographs (200X) are representative of 3–4 experiments with 4–6 representative photos analyzed per treatment.