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. Author manuscript; available in PMC: 2011 Jan 16.
Published in final edited form as: Proteomics. 2008 Nov;8(22):4680–4694. doi: 10.1002/pmic.200800194

Figure 1. Probed Arrays.

Figure 1

Pf protein microarrays were generated as described in Methods. Three individual arrays displaying a subset of targets evaluated in this study were probed with sera as described below, followed by biotinylated secondary and streptavidin PBXL-3, read in a ScanArray 4000 confocal laser scanner, and the signal intensities quantified. Arrays were probed with (A) anti-polyhistidine mouse monoclonal antibody (B) sera from malaria-naïve individuals (pre-immunization specimens from volunteers subsequently immunized with radiation attenuated Pf sporozoites) (C) sera from irradiated sporozoite immunized and infected volunteers post-challenge, or (D) sera from adults naturally exposed to malaria in Africa. Control reactions that lacked vector template (boxes) and reactions of some well characterized Pf antigens (circles) were also spotted.